​羧甲基-茯苓聚糖--上海熹垣生物优势提供
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羧甲基-茯苓

羧甲基-茯苓聚糖--上海熹垣生物优势提供

中文名:羧甲基-茯苓聚糖
英文名:CM-Pachyman
结构式:
 
High purity CM-Pachyman for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Carboxymethylated, (DS ~ 0.2) highly purified pachyman. A soluble/gelatinous substrate for the assay of endo-1,3-β-D-glucanase.
 
CAS NO. 69552-83-6
PREPARATION:
Carboxymethyl pachyman (CM-Pachyman) is prepared by carboxymethylation of highly purified
pachyman with chloroacetic acid. Pachyman is a polymer of 1,3-b-linked D-glucosyl residues.
PROPERTIES OF CM-PACHYMAN:
Degree of carboxymethylation (DS): ~ 0.2.
Colour: tan coloured powder.
Solubility: Forms a colloidal suspension in water at 0.5 % w/v.
Enzyme susceptibility: Readily hydrolysed by endo-1,3-b-glucanase.
DISSOLUTION:
Gradually add 0.5 g of CM-pachyman to 90 mL of vigorously stirring water at 90°C. Continue
stirring for approx. 1 h (until the polysaccharide is completely dispersed). Cool the solution to
room temperature and add 5 mL of sodium acetate buffer (2 M, pH 5.0). Adjust the volume
to 100 mL and store the solution in a well sealed glass container at 4°C. Prevent microbial
infection by adding a few drops of toluene to the storage bottle.
For some 1,3-b-glucanases, a higher pH is require for activity; in these cases, substitute an
appropiate buffer for the acetate buffer (eg. MOPS)
 
 
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